New textbooks at all levels of chemistry appear with great regularity Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to date textbooks The most serious needs occur in fields that are rapidly changing Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry In so me cases the availability of texts in active research areas should help stimulate the creation of new courses....
|Title||:||Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry)|
|Publisher||:||Springer 3rd edition November 19, 1993|
|Number of Pages||:||380 pages|
|File Size||:||980 KB|
|Status||:||Available For Download|
|Last checked||:||21 Minutes ago!|
Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry) Reviews
I am a graduate student that was given the task to purify a protein of interest. We have been trying to purify this protein for a while however since we are using recombinant technology in E. coli it's being expressed as an inclusion body. I received the second edition which was published in the late eighties before recombinant expression was widely used so it's a topic that isn't really touched in the text (although the author anticipated its wide usage in the future).
I find this book, protein purification: principles and practice, is one of the best sources to understand the bases of protein purification techniques. The book describes the mechanisms, chemistry bases, and practical considerations of different protein purification methods. Moreover, the book is written easily for scientists/technicians with background ranging from weak to excellent in biochemistry. If you were new to the field of protein purification, this book will help you to build skills in a very short time since it fills the missing gapes in almost famous protocols by providing very easy background information about each purification procedure. I highly recommend to have it on your book shelf.
This book is great. It provides good, detailed biochemical explanations / theory for a variety of techniques used in protein purification, which are essential for troubleshooting tricky purifications or just getting more knowledgeable in the field.
Working on various biochemical techniques but I have not used them for a while. This is a great refresher and intro for beginners.
In the first chapter of Protein Purification, Scopes notes that more and more biochemical techniques are black boxed, with kits eliminating the need for an understanding of biochemical principles (e.g., "precipitate with Acme Solution B, pour off Solution B, and redissolve precipitant in Solution C"). Scopes notes that with a proper understanding of biochemistry, one can save money on kits and do more purification steps with standard lab materials and reagents. He wrote this book to help biochemistry labs make more informed decisions on protein purification rather than just defaulting on some already familiar kit or protocol.